Cts Nerve Guidance Conduit Material Nerve Defects Properties Regeneration Effect Activity Bioactive Chitosan Conduit Tube Scaffold Inner Cross Sponge Texture

The inner wall of the scaffold was surfaced with C-CM-CTS solution. CM-CTS leaved favorable bioactivities in the composite chitosan-established nerve conduit. An in vitro study of CM-CTS divulged its fulfilling biocompatibility with fibroblast and its inhibition of oxidative damage to Schwann cadres. As the internal filler of the NGC, the lyophilized sponge of C-CM-CTS shewed a longitudinal guidance effect for nerve reconstruction. After  Wellness Industry  in rat sciatic nerve was bridged with the composite bioactive chitosan-free-based nerve conduit, the nerve conduit was able to effectively promote axonal regeneration and worked a positive role in stimulating nerve regeneration and functional recovery. In addition to the functional advantages, which are equal to those of an autograft; the technology for the preparation of this conduit can be put into mass production.

[Effect and mechanism of ultraviolet-cross-linkable chitosan-carbon dots-morin hydrogel treating for rat cartilage injury].OBJECTIVE: To construct a ultraviolet-cross-linkable chitosan-carbon dots-morin (NMCM) hydrogel, observe whether it can repair cartilage injury by in vivo and in vitro experiments, and explore the related mechanism The chitosan was taken to prepare the ultraviolet (UV)-cross-linkable chitosan by aggregating methacrylic anhydride, and the carbon dots by immixing acrylamide.  Dietary Supplements  were mixed and totaled morin solution. After UV irradiation, the NMCM hydrogel was holded, and its sustained release performance was essayed. Chondrocytes were separated from normal and knee osteoarticular (KOA) cartilage tissue donated by patients with joint replacement and identified by toluidine blue staining. The 3rd generation KOA chondrocytes were co-cultured with the morin solvents with densenessses of 12, 25, 50 µmol/L and NMCM hydrogel debased with morin of the same immersions, respectively. The events of morin and NMCM hydrogel on the proliferation of chondrocytes were finded by cell counting kit 8 (CCK-8).

After co-cultured with NMCM hydrogel loaded with 50 µmol/L morin, the level of collagen type Ⅱ (COL-Ⅱ) of KOA chondrocytes was detected by immunofluorescence staining, and the level of reactive oxygen mintages (ROS) was detected by 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) probe. Twenty 4-week old Sprague Dawley rats were selected to construct a articular cartilage injury of right hind limb model, and were randomly divided into two groups ( n=10). The cartilage injury of the experimental group was mended with NMCM hydrogel loaded with 25 µmol/L morin, and the control group was not regaled. At 4 hebdomads after operation, the repair of cartilage injury was honoured by micro-CT and gross observation and marked by the International Cartilage Repair Association (ICRS) general scoring. The cartilage tissue and subchondral bone tissue were keeped by Safranine-O-fast green staining and COL-Ⅱ immunohistochemistry staining and seduced by ICRS histological scoring. The expressions of tumor necrosis factor α (TNF-α), nuclear factor κB (NK-κB), matrix metalloproteinase 13 (MMP-13), and COL-Ⅱ were discovered by Western blot and real-time fluorescence quantitative PCR NMCM hydrogels stretched with different engrossments of morin were successfully retraced. The drug release rate was fast in a short period of time, gradually slowed down after 24 hrs, and the amount of drug release was close to 0 at 96 hrs.

At this time, the cumulative drug release rate gived 88%. Morin with a concentration ≤50 µmol/L had no toxic effect on chondrocytes, and the proliferation of chondrocytes ameliorated under the intervention of NMCM hydrogel ( P<0). NMCM hydrogel stretched with morin could increase the level of COL-Ⅱ in KOA chondrocytes ( P<0) and reduce the level of ROS ( P<0), but it did not reach the normal level ( P<0).